Skip to main content
. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: Mol Immunol. 2018 Oct 20;103:312–321. doi: 10.1016/j.molimm.2018.09.019

Figure 3. DNA from Enhancer 1 activates luciferase transcription.

Figure 3.

(A) The enhancer DNA corresponding to eRNA 134 was subcloned downstream of luciferase under the control of the minimal thymidine kinase promoter (TK). The firefly luciferase vectors were co-transfected with a constitutive Renilla control, pRL-TK, into HEK293 cells. PGL3-TK-A7 is a positive control from near the UBE2VI gene. Construct 172 is a negative control, which encodes a non-coding RNA but is not an eRNA and is not located in the ADAM locus. Inducible firefly luciferase was normalized to the Renilla signal. DNA from Enhancer 1 was able to transactivate TK expression in this system. (B) The Enhancer 1 and 2 eRNAs were coordinately regulated with ADAMDEC1 in primary monocytes. The cells were pretreated by SB203580, JSH-23, SP60025 or U0126 for 30 minutes and then stimulated with LPS for 90 minutes (n=3, error bars in panels denote SD). Symbols indicate statistical significance: * indicates p<0.05, ** p<0.01, and *** p<0.001.