Skip to main content
. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: Mol Immunol. 2018 Oct 20;103:312–321. doi: 10.1016/j.molimm.2018.09.019

Figure 4. The eRNAs regulate transcription.

Figure 4.

(A) Knockdown of eRNA 157 from Enhancer 2 decreased the LPS-induced ADAMDEC1 mRNA but not ADAM28 mRNA. MonoMac6 cells were transfected with the indicated 20-bp phosphorothioate antisense oligonucleotides (ASO) designed to knock down Enhancer 2 eRNA157 (two different ASO) or LACZ for 3 hours and stimulated with or without 1μg/ml LPS for 60 minutes. The eRNAs and mRNAs were measured by qRT-PCR. The ASO effectively knocked down eRNA 157. ADAMDEC1 expression after LPS was diminished although basal expression was not affected. ADAM28 expression was unchanged. (n =3, * p<0.05). (B) LPS-induced mRNA and eRNA kinetic analysis in the primary monocytes. The eRNA and mRNA expression levels were measured by qRT-PCR at various time points and normalized to 18S (mRNAs) or ACTB (eRNAs) (n=3, error bars indicate SD).