Table 3.

Strain specific physiological parameters of the different glyco-engineered P. pastoris Mut^S strains expressing HRP C1A as model enzyme at 30 °C

	wt [12]	∆OCH1 [12]	SuperMan5
max. µGly (h−1)	0.31	0.20	0.28 ± 0.03
YX/Gly (Cmol Cmol−1)	0.41	0.54	0.55 ± 0.03
${\text{Y}}_{{\text{CO}}_2/\text{Gly}}$ (Cmol Cmol−1)	0.64	0.44	0.47 ± 0.02
Δtimeadapt (h)	19.9	8.90	4.85
qMeOH (mmol g−1 h−1)	0.70	0.43	1.52 ± 0.11
YX/MeOH (Cmol Cmol−1)	0.071	0.046	0.033 ± 0.001
${\text{Y}}_{{\text{CO}}_2/\text{MeOH}}$ (Cmol Cmol−1)	1.02	Constantly decreasing	0.97 ± 0.03
C-balance	1.09	Constantly decreasing	1.01 ± 0.05
qp (U g−1 h−1)	0.77	0.50	1.05 ± 0.05

All results were gathered from a previous batch phase on glycerol, a subsequent MeOH adaptation and dynamic pulsing experiment with MeOH. Values for wt and ∆OCH1 strain were taken from [12]