Fig. 4.

Hierarchical clustering analysis of differentially abundant proteins and cluster expression patterns. a Differentially regulated proteins (n = 2680) in Control, Burned, Limit and Regular were clustered into groups with similar log2 transformed ratio (treatment/control) patterns. The Euclidian distance was chosen to cluster proteins by abundance traits. The 8 protein clusters presenting differential abundance trends were chosen for further analysis. b The graphical representation of the four protein abundance patterns (Pattern 1–4) found in the clusters obtained by the hierarchical clustering analysis (sample in x-axis, fold-change on y-axis). Clusters were assigned to groups with a similar pattern and for each pattern, a list of proteins was generated. 112 proteins were part of pattern 1, 102 proteins were part of pattern 2, pattern 3 had 13 proteins and pattern 4, the largest group, had 2453 proteins