Fig. 1.

CSF1R antagonism depleted microglia in adult and aged BALB/c mice. a Adult (6–8 weeks old) and aged (16–18 months old) male BALB/c mice were provided diets formulated with vehicle or CSF1R antagonist (PLX5622) for 21 d. After 21 d, mice were sacrificed, microglia were Percoll-enriched, and the number of microglia was determined in the brain. b Representative bivariate dot plots of CD45 and CD11b labeling of Percoll-enriched microglia. c Percent of CD11b⁺/CD45^low microglia in the brain of adult and aged mice after 21 days of PLX5622 administration normalized to vehicle controls (n = 5 mice / group). In the same mice, the hippocampus was microdissected, and mRNA levels of key microglial signature genes were assessed by nanoString nCounter analysis (n = 3–4 mice / group). d PCA plot showing unsupervised clustering of treatment groups by both PLX5622 (PC1) and age (PC2). e Heat map of hippocampal mRNA signature. Bars represent the mean ± SEM. Means with * are different from age-matched vehicle controls (P < 0.05)