Figure 4.

TNF‐α ablation promoted myoblast fusion. (a) QPCR analysis showed no detectable TNF‐α expression in primary myoblasts isolated from TNF‐α‐null mice. *Significant difference from wild‐type myoblasts at p < 0.05. N = 6 per data set. (b) TNF‐α‐null myotubes have more nuclei compared to wild‐type. *Significant difference from wild‐type myotubes at p < 0.05. Results are from three independent experiments. N = 6 per data set. (c) QPCR analysis of primary myoblasts isolated from wild‐type and TNF‐α‐null mice showed no difference in Pax7, MyoD, or myogenin mRNA. Values normalized to myoblasts isolated from wild‐type mice. N = 5 per data set