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. Author manuscript; available in PMC: 2018 Nov 28.
Published in final edited form as: Anal Chem. 2018 Jan 9;90(3):2001–2009. doi: 10.1021/acs.analchem.7b04089

Figure 1.

Figure 1.

Typical 800 MHz (cryo-probe) 1D CPMG 1H NMR spectrum of human serum obtained after protein precipitation using methanol (1:2, v/v, serum/methanol) with expanded regions and peak annotations for the 30 metabolites, which were quantitated using NMR to guide their absolute quantitation using MS.