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ISO and SEVO are well tolerated in flies. (A) Mixed-sex, 0-7 or 1-8 day old w1118 flies were exposed to different doses (%hr) of ISO or SEVO and the percent of flies that died within 24 hrs (percent mortality) was measured. Flies not exposed to anesthetic (CTRL) were examined to establish the baseline percent mortality. (B) %h values from panel A for ISO and SEVO were normalized to their respective EC50 values (ISO, 0.41±0.03 and SEVO, 0.68±0.05), as determined by Olufs et al.8
