Appendix 1–figure 1.

(A) ATAC-seq insertional density at the biotin gene locus of ministat-aged cultures. (B) RNA-seq analysis of biotin genes at increasing ages from this manuscript and Janssens et al. (C) Biotin synthesis from DAPA in yeast. (D) Growth curves of DBY12000 in different media. AS and BW are two separate media preparations lacking biotin.