Figure 7:

In vitro recreation of an in vivo tumor. (a) two-photon image showing human colon carcinoma vasculature in mice at day 0 reproduced from Tong, R.T., Y. Boucher, S.V. Kozin, F. Winkler, D.J. Hicklin, and R.K. Jain. Vascular normalization by vascular endothelial growth factor receptor 2 blockade induces a pressure gradient across the vasculature and improves drug penetration in tumors. Cancer Res 64, 3731, 2004, with permission from AACR [60]. Circled region indicates areas difficult to recreate in vitro. (b) Engineered microfluidic tumor microenvironment capturing the geometry of an in vivo tumor, scale bar is 100 μm. (c) Obtained velocity magnitude inside in vivo tumor microenvironment microvascular using FEM simulations, scale bar is 500 μm.