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. 2018 Oct 20;8(20):e3061. doi: 10.21769/BioProtoc.3061

Table 1. Chronic Mild Stressors (from 4-7 weeks).

Week 1 Day 1 Day 2 Day 3 Day 4 Day 5 Day 6 Day 7
8:30 am Water deprivation Damp Bedding

Tube

Restraint

Water Deprivation Damp Bedding

Tube

Restraint

Water Deprivation
5:30 pm

Tail

Suspension

Food Deprivation Strobe Light

Tail

Suspension

Food Deprivation Strobe Light Anhedonia Test
WEEK 2 DAY 8 DAY 9 DAY 10 DAY 11 DAY 12 DAY 13 DAY 14
8:30 am

Tail

Suspension

Tube

Restraint

Water Deprivation Damp Bedding

Tube

Restraint

Water Deprivation Damp Bedding
5:30 pm Food Deprivation Strobe Light

Tail

Suspension

Food Deprivation Strobe Light

Tail

Suspension

Anhedonia Test
WEEK 3 DAY 15 DAY 16 DAY 17 DAY 18 DAY 19 DAY 20 DAY 21
8:30 am Food Deprivation Water Deprivation Damp Bedding Damp Bedding Empty Bottle Water Deprivation Damp Bedding
5:30 pm Strobe Light

Tail

Suspension

Tube

Restraint

Food Deprivation Strobe Light

Tail

Suspension

Anhedonia Test

WEEK 4 DAY 22 DAY 23 DAY 24 DAY 25 DAY 26 DAY 27 DAY 28
8:30 am

Tail

Suspension

Tube

Restraint

Water Deprivation Damp Bedding

Tube

Restraint

Water Deprivation Anhedonia Test
5:30 pm Food Deprivation Strobe Light

Tail

Suspension

Food Deprivation Strobe Light

Tail

Suspension

Sacrifice