KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Streptavidin IRDye 800 CW | LiCor (1:10000) | P/N 926-32230 |
| mouse anti-b-Galactosidase | DSHB (1:100) | JIE7 |
| mouse anti-b-Galactosidase (mAb40-1a) | DSHB (1:100) | ID: AB_2314509 |
| rat anti-GFP | Nacalai Tesque (1:500) | Cat# GF090R; RRID: AB_2314545 |
| rabbit anti-active-Dcp1 | Cell Signaling Technology (1:50) | Cat #9578 |
| mouse anti-discs large (4F3) | DSHB (1:50) | ID: AB_528203 |
| Mouse anti-dSparc | Portela et al., 2010 | mAb30A1B4 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Heat inactivated Fetal Bovine Serum | Gibco | 10082139 |
| Schneider's Drosophila Medium | Gibco | 21720024 |
| Trizol | Ambion | 15596-026 |
| Cycloheximide | Sigma | C1988 |
| Critical Commercial Assays | ||
| Click-iT® Plus OPP Alexa Fluor® 594 Protein Synthesis Assay Kit | Thermofisher | C10457 |
| Maxiscript T7 Transcription kit | Ambion | AM1312 |
| Deposited Data | ||
| mRNA-Seq raw data | This paper | GSE112864 |
| DESEQ2 analysis wildtype vs RpS17/+ | This paper | GSE112864 |
| DESEQ2 analysis wildtype vs RpS3/+ | This paper | GSE112864 |
| DESEQ2 analysis wildtype vs RpS3/+ Xrp1/+ | This paper | GSE112864 |
| DESEQ2 analysis RpS3/+ vs RpS3/+ Xrp1/+ | This paper | GSE112864 |
| Experimental Models: Organisms/Strains | ||
| D. melanogaster mutation Tor[DeltaP] | (Zhang et al., 2000) | FBal0120586 |
| D. melanogaster transgenic strain P{TRiP.JF01761}attP2 (mycRNAi) | (Ni et al., 2011) | FBti0114534 |
| D. melanogaster transgenic strain P{y[+t7.7] v[+t1.8]=TRiP.HMS00811}attP2 (Gadd34RNAi) | Bloomington (Ni et al., 2011) | FBti0140523 |
| D. melanogaster mutation Xrp1m2-73 | Lee et al., 2016 | N/A |
| D. melanogaster mutation Xrp1Δ1 | This paper | N/A |
| D. melanogaster mutation RpS3 | (Burke and Basler, 1996) | N/A |
| D. melanogaster transgenic strain UAS-Xrp1-Flg-CDS2 | Tsurui-Nishimura et al., 2013 | N/A |
| D. melanogaster transgenic strain P{PZ}Xrp1[02515] | Spradling et al., 1999 | Flybase: FBal0009448 |
| D. melanogaster transgenic strain P{GUS-p53.259H} | Brodsky et al., 2000 | Flybase: FBti0023695 |
| D. melanogaster transgenic strain P{GUS-p53.Ct} | Brodsky et al., 2000 | Flybase: FBti0023694 |
| D. melanogaster transgenic strain en-Gal4 | (Neufeld et al., 1998) | RRID:BDSC_6356 |
| D. melanogaster mutant strain Df(3R)Exel6181 | Exelixis, Inc. | Flybase: FBab0038236 |
| D. melanogaster mutant strain Df(3R)Exel6182 | Exelixis, Inc. | Flybase: FBab0038237 |
| D. melanogaster mutant strain Df(3R)Exel6187 | Exelixis, Inc. | Flybase: FBab0038242 |
| D. melanogaster strain Df(1)su(s)R194 (deleting RpL36 gene) | (Duffy et al., 1996) | FBab0024817 |
| D. melanogaster strain M(2)56f (mutating RpS18) | Laboratory of Y. Hiromi | FBal0284387 |
| D. melanogaster, RpS17 mutation M(3L)67C4 | Morata and Ripoll, 1975 | Flybase: FBal0011935 |
| D. melanogaster mutant for RpL27A Df(2L)M24F11 | Marygold et al., 2007 | Flybase: FBab0001492 |
| D. melanogaster. RpS12 mutation rpS12G97D | Tyler et al., 2007 | FBal0193403 |
| D. melanogaster UAS-rpS12 transgenic strain | (Kale et al., 2018) | FBal0337979 |
| D. melanogaster. RpL36+ transgenic strain | Tyler et al., 2007 | FBal0193398 |
| D. melanogaster. act>CD2>Gal4 expressing strain | Pignoni and Zipursky, 1997) | N/A |
| Oligonucleotides | ||
| See Table S4 | ||
| Software and Algorithms | ||
| ImageJ | (Schneider et al., 2012) | v1.44j |
| Photoshop CS5 | Adobe | V12.0.1 |
| Other | ||
| Biodyne B Nylon membrane | Thermofisher | 77016 |
| ULTRAhyb-Oligo buffer | Ambion | AM8663 |
| Odyssey Blocking buffer (PBS) | Li-COR | 927-40003 |
| Biotin-16-UTP | Roche | 11388908910 |
| RNA Sample Loading Buffer | Sigma | R4268-5VL |