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. Author manuscript; available in PMC: 2018 Nov 28.

Published in final edited form as: Intern Med Rev (Wash D C). 2018 Oct;4(10):10.18103/imr.v4i10.768. doi: 10.18103/imr.v4i10.768

Figure 2. — Renal extracts (20–35 ug) were resolved on SDS-PAGE gels and immunoblotted. (A) Representative DRP1 western blots showing distinct protein bands of DRP1 (~75 kDa) in Sham and CLP kidneys. Densitometry evaluation of each blot (normalized to actin) are shown below. (B) Densitometry evaluation of MFN1 and MFN2 in both genders and both CLP models. (C) Densitometry evaluation of OPA1 L (long form) and OPA1 S (short form) in both genders and both CLP models. Actin was used as a loading control for all western blots. Values were expressed as Mean ± SEM (n =4). *P< 0.05 compared to the corresponding Sham group; **P < 0.05 compared to the corresponding CLP non-nephrectomy group.

Figure 2. — Renal extracts (20–35 ug) were resolved on SDS-PAGE gels and immunoblotted. (A) Representative DRP1 western blots showing distinct protein bands of DRP1 (~75 kDa) in Sham and CLP kidneys. Densitometry evaluation of each blot (normalized to actin) are shown below. (B) Densitometry evaluation of MFN1 and MFN2 in both genders and both CLP models. (C) Densitometry evaluation of OPA1 L (long form) and OPA1 S (short form) in both genders and both CLP models. Actin was used as a loading control for all western blots. Values were expressed as Mean ± SEM (n =4). *P< 0.05 compared to the corresponding Sham group; **P < 0.05 compared to the corresponding CLP non-nephrectomy group.