Fig. 4. β-catenin steady state protein levels were increased at late stages of productive infection.
HLF cells were infected with HSV-1 (Panels A and B) or UV-inactivated HSV-1 (Panels C and D) at a MOI of 1. Vero cells were infected with HSV-1 (Panel E and F) or UV-inactivated HSV-1 (Panel G and H) at a MOI of 1. Mock-infected cells were included as a control. At the indicated times after infection, total cell lysate was prepared and Western blots performed using specific antibodies that recognize β-catenin, VP16, and Tubulin as described in the materials and methods.
Panel B, D, F and H: The band intensity of β-catenin and Tubulin was quantified with software Image J. Levels of β-catenin were compared to Tubulin and the changes in β-catenin expression are presented. These results are representative of three independent studies.