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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Nat Cell Biol. 2018 Oct 22;20(11):1303–1314. doi: 10.1038/s41556-018-0215-z

Figure 1. CRAD inactivation in CRC.

Figure 1.

a, Oncomine analysis of CRAD expression in human cancers.

b, GEO (GDS2947) analysis of CRAD expression in adjacent normal tissues vs. colorectal adenoma tissues. n=32 patients; probes: 227231_at and 227230_s_at.

c, IHC of CRAD in normal colon and colorectal adenocarcinoma. Images are representative of 14 normal colon and 38 CRC samples.

d and e, CRAD expression in IECs and CRC cells. qRT-PCR (d; n=3 independent experiments) and IB (e) analyses. The representative images are shown from three independent IB experiments.

f, Genetic alteration of CRAD. cBioportal datasets: Genentech 2012 (n=72 patient samples); TCGA pub 2012 (n=212 patient samples); TCGA provisional (n=220 patient samples); DFCI 2016 (n=619 patient samples).

g and h, COSMIC analysis of CRAD mutations in CRC. n values indicate patient sample number.

Scale bars indicate 50μm; Error bars: mean ± S.D.; NS: not significant (P>0.05); Two-sided unpaired t-test.