REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-Ki-67	Abcam	Cat#ab15580; RRID:AB_2088164
Rabbit polyclonal anti-SDF1	Abcam	Cat#ab25117; RRID:AB_2088164
Rabbit monoclonal anti-Ezh2 XP (clone D2CP)	Cell Signaling Technology	Cat#5246S; RRID:AB_10694683
Rabbit polyclonal anti-αSMA (clone 1A4)	Abcam	Cat#Ab7817; RRID:AB_262054
Rabbit polyclonal anti-Keratin 14 (clone Poly19053)	BioLegend	Cat#905304; RRID:AB_2616896
Rabbit polyclonal anti-Keratin 10 (clone Poly19054)	BioLegend	Cat#905404; RRID:AB_2616955
Rabbit polyclonal anti-Loricrin (clone Poly19051)	BioLegend	Cat#905104; RRID:AB_2616895
Rabbit polyclonal anti-β-actin-HRP (C-11)	Santa Cruz Biotechnology	Cat#sc-1615; Lot#G2313, A3105; RRID:AB_630835
Donkey Anti-Rabbit IgG H&L (Alexa Fluor 488)	Abcam	Cat#ab150073; RRID:AB_2636877
Rabbit monoclonal anti-Tri-Methyl-Histone H3 (Lys27) (clone C36B11)	Cell Signaling Technology	Cat#9733; RRID:AB_2616029
Rabbit polyclonal anti-trimethyl-Histone H3 (Lys4)	Millipore	Cat#07473; RRID:AB_1977252
ChIPAb+ EZH2 – ChIP Validated Antibody and Primer Set (Clone AC22)	Millipore	Cat#17-662; RRID:AB_1977568
Biological Samples
Normal Adult Primary Human Keratinocytes	Penn Skin Biology and Diseases Resources-based Center (SBDRC)	https://dermatology.upenn.edu/sbdrc/
Normal Human Primary Keratinocytes	Penn SBDRC	https://dermatology.upenn.edu/sbdrc/
Microarray data for baseline and wounded human skin	Stone et al., 2017	GEO: GSE84571
Microarray data for baseline and wounded human skin	Nuutila et al., 2012	GEO: GSE28914
Microarray data for baseline and wounded human skin	Nuutila et al., 2013	GEO: GSE33169
Chemicals, Peptides, and Recombinant Proteins
Meloxicam Solution for Injection (5mg/mL)	Putney	NDC: 26637-621-01; Lot#GI60154
Buprenorphine Hydrochloride, Injection (0.3mg/mL)	Butler Schein Pharmaceuticals	NDC: 42023-179-05; Lot#809762
TRI Reagent	Zymo Research	Cat#R2050-1-200; Lot#ZRC184119; ZRC182093
Lysing Matrix D (2mL Tube)	MP Biomedicals	Cat#116913050; Lot#104291
Maxima Reverse Transcriptase	Thermo Scientific	Cat#EP0741
Histone Methyltransferase EZH2 Inhibitor, DZNep	Millipore	Cat#252790; Lot#2704823; Lot#D00157396
Maxima Reverse Transcriptase	Thermo Scientific	Cat#EP0741
Doxycycline Grain-Based Rodent Diet (6g/kg)	Bio-Serv	Cat#14-727-450
Tamoxifen	Sigma	Cat#T5648-1G
Critical Commercial Assays
Direct-zol RNA MicroPrep	Zymo Research	Cat#R2062; Lot#ZRC188234
DAB-Peroxidase IHC Kit	Abcam	Cat#ab80436
Mouse Acta2 Taqman Probe	Applied Biosystems	Cat#Mm00725412_s1; Lot#1501761
Mouse Beta-Actin (Actb) Taqman Probe	Applied Biosystems	Cat#Mm00607939_s1; Lot#P151010-002
Mouse Cxcl12 Taqman Probe	Applied Biosystems	Cat#Mm00445553_m1; Lot#1617669
Mouse Cdkn1a Taqman Probe	Applied Biosystems	Cat#Mm00432448_m1; Lot#1246220
Mouse Ezh2 Taqman Probe	Applied Biosystems	Cat#Mm00468464_m1; Lot#833569
Human Beta-Actin (Actb) Taqman Probe	Applied Biosystems	Cat#Hs99999903_m1; Lot#P150812-005 H11
Human Cxcl12 Taqman Probe	Applied Biosystems	Cat#H203676656_mH; Lot#P170224-001
Human Ezh2 Taqman Probe	Applied Biosystems	Cat#H201016789_m1; Lot#P130125-005
Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit	R&D Systems	Cat#MCX120; Lot#P134406
cOmplete Mini, EDTA-free Protease Inhibitor	Roche	Cat#11836170001; Lot#13538100
Dynabeads Protein A	Invitrogen	Cat#10002D; Lot#00357670
eBioscience 1X RBC Lysis Buffer	Invitrogen	Cat#00-4333-57
Liberase TL Research Grade	Roche	Cat#5401020001
7-AAD Viability Staining Solution	BioLegend	Cat#420403
Anti-Mouse Ig, κ/Negative Control Compensation Particles Set	BD Biosciences	Cat#51-90-9001229; Lot#6236953
PolyScreen PVDF Hybridization Transfer Membrane	Perkin Elmer	Cat#NEF1002001; Lot#507409
Luminata Crescendo Western HRP Substrate	Millipore	Cat#WBLUR0100; Lot#160263
4-5% Mini-PROTEAN TGXPrecast Protein Gel	Bio-Rad	Cat#4561083
Experimental Models: Cell Lines
293T Cells	ATCC	CRL-3216; RRID:CVCL_0063
Experimental Models: Organisms/Strains
Mouse: C57/BL6 “Black 6”	The Jackson Laboratory	JAX: 000664; RRID:IMSR_JAX:000664
Mouse: B6(FVB)-Cxcl12tm1-1Link/J (“Cxcl12fl/fl”)	The Jackson Laboratory	JAX: 021773; RRID:IMSR_JAX:021773
Mouse: B6.129(Cg)-Gt(ROSA)26Sortm3(ACTB-tdTomato,-EGFP)Luo/J (“mTmG”)	The Jackson Laboratory	JAX: 007676; RRID:IMSR_JAX:007676-UCD
Mouse: B6;129S2-Cdkn1atm1Tyj/J (“Cdkn1a−/−“)	The Jackson Laboratory	JAX: 003263; RRID:IMSR_JAX:003236
Mouse: STOCK Tg(KRT14-cre/ERT)20Efu/J (“K14CreER+”)	The Jackson Laboratory	JAX: 005107; RRID:IMSR_JAX:005107
Mouse: K5rtTA; TetOCre	Sarah Millar	N/A
Oligonucleotides
ChIP Mouse Cxcl12 promoter FWD: CTGTTTCGTCTCTCAGGTTCTT	Integrated DNA Technologies	N/A
ChIP Mouse Cxcl12 promoter REV: GCTGGGTCGTAGAGCTTAATG	Integrated DNA Technologies	N/A
ChIP Mouse Cxcl12 Transcription Start Site FWD: CTGTTTCGTCTCTCAGGTTCTT	Integrated DNA Technologies	N/A
ChIP Mouse Cxcl12 Transcription Start Site REV: GCTGGGTCGTAGAGCTTAATG	Integrated DNA Technologies	N/A
ChIP Mouse Cxcl12 upstream FWD: CAACATCTCCTTCTTTCCCTACC	Integrated DNA Technologies	N/A
ChIP Mouse Cxcl12 upstream REV: CTACCCTCCAACAAGCATTCA	Integrated DNA Technologies	N/A
ChIP Mouse Cxcl12 −20kb control FWD: GCCTGGAGAACCTTCTATCTTAAC	Integrated DNA Technologies	N/A
ChIP Mouse Cxcl12 −20kb control REV: GTCCACATGCAAATCTTCACAC	Integrated DNA Technologies	N/A
Recombinant DNA
Human EZH2: 2146 CGGCTCCTCTAACCATGTTA pLKO_TRC005	The RNAi Consortium	TRCN0000039040
Software and Algorithms
FlowJo	Treestar	RRID: SCR_008520; https://www.flowjo.com/
Other
2mm mechanical punch	Roboz	Cat#65-9906
1.5mm biopsy punch	Acuderm	Cat#P1550
6mm biopsy punch	Delasco	Cat#DBP-6
Silicone wound splints	Grace Biolabs	SKU: 476687
Medium 154	GIBCO	Cat#M154500
Human Keratinocyte Growth Supplement	GIBCO	Cat#S0015
Keratinocyte-SFM (1×)	GIBCO	Cat#17005042
DMEM 4.5g/L glucose, L-glutamine, sodium pyruvate	Corning	Cat#10-013-CV