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. Author manuscript; available in PMC: 2018 Nov 28.
Published in final edited form as: J Cell Physiol. 2016 Mar 9;231(11):2452–2463. doi: 10.1002/jcp.25357

Figure 6. FPP elevated by ZGA treatment suppresses the expression of K6 promoter through co-repressor complex involving β-catenin.

Figure 6

A. CAT assay after co-transfection of human keratinocytes with K6-CAT promoter. β-catenin enhances repression of K6 in the presence of both ZGA and DEX, acting as a co-repressor of GR. In the presence of accumulated FPP (ZGA), β-catenin-mediated K6 co-repression is further enhanced by CARM-1. B. Graphs represent quantitative CAT assay after co-transfection of human keratinocytes with GRE-CAT showing that unlike DEX, ZGA does not recruit CARM-1 or β-catenin to GRE. The data are presented as relative CAT activity, normalized to total protein. Values represent the mean ± S.D. from three independent experiments. C. Dex and ZGA regulate common and distinct β-catenin transcriptional targets which are predicted to collectively affect cellular movement. Black = upregulation, White = downregulation. 34 of the 42 ZGA and/or DEX-regulated β-catenin transcriptional targets are known to affect cell movement. Lines represent literature-supported findings in the Ingenuity Knowledge Base. Figure was generated using Ingenuity Pathway Analysis.