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. Author manuscript; available in PMC: 2018 Nov 28.
Published in final edited form as: J Cell Physiol. 2016 Mar 9;231(11):2452–2463. doi: 10.1002/jcp.25357

Figure 7. FPP targets the K6 promoter through co-repressor Grip1.

Figure 7

A. Transfection experiments of HEK with the K6-CAT reporters are shown. FPP treatment results in further suppression of K6 promoter in the presence of Grip1. B. GRIP1 DNA binding mutant – NRBmII/III abolishes K6 suppression mediated by FPP. Graphs represent quantitative CAT assay after co-transfection of human keratinocytes; values represent the mean ± S.D. from three independent experiments. C. ChIP with Grip1 antibody. HEK were either untreated (−) or treated with 1μM DEX or 10 μM FPP. Same amounts of genomic DNA (input) was used in each treatment. K6 promoter sequences were amplified by PCR after immunoprecipitation with anti-GRIP1 antibody or IgG.