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. Author manuscript; available in PMC: 2018 Nov 28.
Published in final edited form as: Clin Immunol. 2017 Aug 16;183:132–141. doi: 10.1016/j.clim.2017.08.010

Figure 4. 24h Proteinuria and C3 and IgG Deposition in NZM WT vs. NZM ERα−/− mice.

Figure 4

A) 24h proteinuria was assessed by albumin ELISA. At 18 weeks, mild proteinuria was observed in most groups although NZM WT OVX mice were protected from early proteinuria. B) By 32 weeks (or the terminal time point), most NZM mice had clinically significant proteinuria with the exception of intact NZM ERα−/− mice, which were protected. E2 exacerbated proteinuria, even in NZM ERα−/− mice which had significantly more proteinuria than intact NZM WT or NZM ERα−/− mice. C) C3 and IgG deposition were assessed via immunofluorescence after incubating slides with sheep anti-mouse C3 or rabbit anti-mouse IgG antibody. Slides were scored on a 0–3 scale by a blinded investigator. Almost all mice had moderate to high C3 and IgG deposition, and there were no significant differences in immune complex deposition among the groups, although NZM ERα−/− mice trended towards reduced C3 deposition.