Figure 2.
Mutagenesis of two TM6 phenylalanines reveals a role for bulky nonpolar residues in maintaining channel conductance and the stability of the open state. (a) Portions of the oocyte periphery 5 days after injection with the indicated MSL10‐GFP variant cRNA. Scale bar is 100 μm. (b) Examples of traces at −100 mV membrane potential. 0.5‐s fragments of 5‐s records of channel activation by symmetric pressure ramps are shown. (c) Current/voltage curves for the indicated MSL10 variants. Each data point is the average current from 3 to 9 patches in 60 mM MgCl2, 4 mM HEPES. Error bars indicate standard deviation but are obscured by the symbols. Gray background indicates voltages where the current produced by MSL10 F553L differed significantly from wild‐type MSL10, p < 0.001 (Student's t test)