Figure 6.

MSL10 mechanosensitive channel activity is not required for death signaling in planta. (a) Localization of MSL10‐GFP variants transiently expressed in tobacco epidermal pavement cells. Images were taken 5 days after infiltration. Infiltrations with P19 alone were used as a negative control. Scale bar is 40 μm. Numbers in the lower left‐hand corner indicate the voltage setting of the PMT detector when imaging. (b) Examples of cell viability assays. Tobacco leaves expressing MSL10‐GFP variants were dual stained with FDA and PI; cells were scored as dead if they had a PI‐stained nucleus (indicated by asterisks) or a disappearing vacuole, evidenced by spreading of cytoplasmic signal (arrowhead). Scale bar is 50 μm. (c) Percentage of dead cells quantified from dual staining of 20 leaves (P19 and MSL10) or 10 leaves (MSL10 variants) from multiple infiltration experiments. Error bars indicate standard error. Statistical differences were assessed by one‐way ANOVA and Scheffe's test; groups with the same letter did not significantly differ from each other (p > 0.05)