Figure 3. PHF6 C99F Mice Exhibit Seizure Susceptibility and Increased Neuronal Excitability.

(A) Time to generalized seizure upon PTZ induction. Unpaired t test, *p < 0.05.

(B) Racine scale scoring of animal behavior upon kainic acid seizure induction. Two-way repeated-measures ANOVA. *p < 0.05.

(C) Current-clamped layer II stellate cells in acute slices of the entorhinal cortex were depolarized to potentials from −55 to −44 mV with a step of 1 mV via automatic current injection to induce action potential (AP) firing. *p < 0.05, **p < 0.01; chi-square test within corresponding potentials.

(D) Sample traces of spontaneous AP firing recorded at −50 mV in control and C99F neurons.

(E) AP firing rate was measured as number of AP fired during a 20-s period. *p < 0.05, t test.

(F) APs were evoked by a ramp current injection (0.15 pA/ms, lower traces). Representative AP traces are shown (upper traces). Only the first AP was used to estimate the AP threshold (arrows).

(G) Summary of the data in (F) revealed that AP threshold is decreased in C99F mice. **p < 0.01, t test.

(H) Average rest membrane potential (RMP).

(I) Membrane capacitance.

(J) Input resistance.

(K) Capacitance-normalized input resistance.

**p < 0.01, t test, unless specified. Data are presented as means ± SEMs.