REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit anti PHF6	Novus Biologicals	NB100-68262; RRID: AB_1109819
Rat anti CTIP2	Abcam	Ab18465; RRID: AB_2064130
Mouse anti 14-3-3β	Santa Cruz	Sc-1657; RRID: AB_626618
Rabbit anti FLAG	Sigma	F7425; RRID: AB_439687
Alexa-Fluro 488-conjugated Avidin	Thermo Fisher Scientific	A21370
Alexa-Fluro 488-conjugated goat anti rabbit	Abcam	Ab150077; RRID: AB_2630356
Cy3-conjugated goat anti rat	Invitrogen	A10522
Goat anti-mouse HRP	Calbiochem	401253; RRID: AB_437779
Goat anti-rabbit HRP	VWR	80056-796
Chemicals, Peptides, and Recombinant Proteins
Kainic Acid	Sigma	K2389
Pentylenetetrazole	Sigma	P6500
Biocytin	Sigma	B4261
Hoechst 33258 Solution	Sigma	94403
T7 Endonuclease I	NEB	M0302S
BbsI	NEB	R0539S
Critical Commercial Assays
Q5® Site-Directed Mutagenesis Kit	NEB	E0554S
Deposited Data
Raw RNaseq data	This paper	GSE119973
Experimental Models: Cell Lines
Human: HEK293T cells	ATCC	CRL-3216
Experimental Models: Organisms/Strains
Mouse: PHF6 C99F knock-in:B6.PHF6(nt.296G > T)	Laboratory of Dr. Azad Bonni	N/A
Mouse: PHF6 knockout:B6	Laboratory of Dr. Azad Bonni	N/A
Oligonucleotides
sgRNAs (5′-GTGATGTGAAAACCTGCCAC-3′)	IDT	N/A
Off-target primers: 1F (5′- TTGGGAGCTTGTGGGACCTG-3′)	IDT	N/A
Off-target primers: 1R (5′- CTTGGCTCCTGATGACGGCA-3′)	IDT	N/A
Off-target primers: 2F (5′- TTTGGAAACTTTCCGGTTGGGGG –3′)	IDT	N/A
Off-target primers:2R (5′-GGCACTTGCTGTCCATAATGCTG-3′)	IDT	N/A
Off-target primers: 3F (5′- GGAAGTGGTCCATGCCACTG-3′)	IDT	N/A
Off-target primers: 3R (5′-GCCAAAGCTCGTTCTGAAGTGATG-3′)	IDT	N/A
Off-target primers: 4F (5′- CTTTAACCAGGTACTAAGTGTGACAGTGTG –3′)	IDT	N/A
Off-target primers: 4R (5′- GGAATGGACGTGGCCTTTAAAGC –3′)	IDT	N/A
Off-target primers: 5F (5′- GTTGGCAACCCAGAGAGACA –3′)	IDT	N/A
Off-target primers: 5R (5′-ACCACCAGTGTTGTGTCCAG-3′)	IDT	N/A
Off-target primers: 6F (5′-ACCTGAGTAACCAAGTCCACAC-3′)	IDT	N/A
Off-target primers: 6R (5′-TTACAGCAGACCTCTAGGGATT-3′)	IDT	N/A
Off-target primers: 7F (5′- CCACTGTGGTTAGACTCCCTC –3′)	IDT	N/A
Off-target primers: 7R (5′- AGACATGCCTGTAGCAGACT –3′)	IDT	N/A
Upstream F1 (5′- GAACAAGGCGTTTGGCAACCAGG-3′)	IDT	N/A
Upstream R1 (5′- GGAGCTGAGCTAGATACAACTG –3′)	IDT	N/A
Middle F2 (5′-GTATCCAGCAAGTTCTGGATTGC-3′)	IDT	N/A
Middle R2 (5′-GGAAAAACTTAGTAGAGGACTAC-3′)	IDT	N/A
Downstream F3 (5′-CTGCACATAACTCGGAAGGTATG-3′)	IDT	N/A
Downstream R3 (5′-GGTACACAGACATACCCGCAGG-3′)	IDT	N/A
Recombinant DNA
pHAGE-NTAP-del279-365-PHF6	Lab: Prof. Dr. Azad Bonni	N/A
pHAGE-NTAP-R342X-PHF6	Lab: Prof. Dr. Azad Bonni	N/A
pHAGE-NTAP-H229R-PHF6	Lab: Prof. Dr. Azad Bonni	N/A
pHAGE-NTAP-C45Y-PHF6	Lab: Prof. Dr. Azad Bonni	N/A
pHAGE-NTAP-C99F-PHF6	Lab: Prof. Dr. Azad Bonni	N/A
pHAGE-NTAP-C305X-PHF6	Lab: Prof. Dr. Azad Bonni	N/A
pHAGE-NTAP-R257G-PHF6	Lab: Prof. Dr. Azad Bonni	N/A
pHAGE-NTAP-PHF6	Lab: Prof. Dr. Azad Bonni	N/A
PX330	addgene	Addgene #42230
Software and Algorithms
R	Open Source, Software	R Project for Statistical Computing, RRID:SCR_001905
GraphPad Prism 6.0	GraphPad Software	https://www.graphpad.com/ RRID: SCR_002798
EdgeR	Open source	RRID:SCR_012802
DEseq2	Open source	RRID:SCR_015687
BrainSpan	Allen Institute for Brain Science	RRID:SCR_008083
HUGO multi-symbol checker	Open source	https://www.genenames.org/cgi-bin/symbol_checker
SPSS (v.2.5)/Systat	SPSS software	RRID:SCR_002865
Adobe Photoshop	Adobe	RRID:SCR_014199
COSMID	Cradick et al., 2014	https://crispr.bme.gatech.edu/
CRISPOR/v.4.4	Haeussler et al., 2016	http://crispor.tefor.net/
Fiji	ImageJ software	https://imagej.nih.gov/ij/