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. 2018 Nov 28;13(11):e0207303. doi: 10.1371/journal.pone.0207303

Fig 5. In vivo osteogenic effects of C. pallidum extract–caudal fin model.

Fig 5

(a) Schematic detailing the method used for the caudal fin model. (b) Histogram showing pixel intensity frequencies in classes 7–13 and 14–20. (c) Picture of alizarin red-S stained caudal fin (red channel) from a control individual 120 hpa. (d) Picture of stained caudal fin from an individual treated with C. pallidum at 1.56 μg per mg fish weight (same for all treated individuals), 120 hpa. (e) Average percentage frequency (n = 5) for control and C. pallidum treated individuals within pixel intensity classes of 7–13 and 14–20. (f) Degree of osteogenesis at 120 hpa. (g) Degree of regeneration at 120 hpa. * indicates a statistically significant difference (p<0.05) compared to the relevant control.