Fig 2. Cytokine secretion by RORA knockout cells.

Deletion of RORA led to increase in basal and LPS-induced cytokine secretion. A. IL-1β has the biggest increase of approximately 2500 folds, B. IL-6 levels increases nearly 15 folds, C. TNF levels increases almost 900 folds, D. IL-1α levels increases approximately 50 folds and E. IL-18 levels increases 5 folds in deletion cells. 2.5×10⁵ cells in 24-well plates were differentiated using 50 ng/ml PMA for 48 hours. Stimulation was performed after 24 hours of rest in PMA-free media with 10 ng/ml LPS for 2, 4, 6 and 24 hours. Cytokines quantification was performed using multiplex cytokine assay on the supernatant. Graphs are representatives for at least three independent experiments. Statistical analysis was calculated using ANOVA test. Stars indicate significance of differences between control and knockout cells after post hoc Bonferroni correction.