Table 1. List and main characteristics of strains used for EV preparation.
Phylogenetic groups | Species/subspecies | Strains | Main hosts | Clinical signs | CFU/ml in m-PPLOa | EV preparation | |||
---|---|---|---|---|---|---|---|---|---|
0h | 24h | 48h or 96h | Densityb | Cell contaminationc | |||||
Phytoplasma, Acholeplasma | A. laidlawii | PG8T | Vertebrate and invertebrate | none | 2.1+/-0.6x109 | 1.0+/-0.8x109 | (96h) 3.5+/-2.4x107 | + | 56/1.8x1010 |
Spiroplasma | M. mycoides subsp. mycoides | Afadéd | Cattle | Contagious bovine pleuropneumonia | 9.8+/-1.7x108 | 3.2+/-0.8x109 | (96h) 4.9+/-4.3x107 | ++ | 0/2.5x1010 |
M. mycoides subsp. capri | PG3T | Goat | Contagious agalactia | 8.4+/-0.4x108 | 4.8+/-0.9x109 | (48h) 3.4+/-0.0x108 (96h) 9.5+/-2.2x104 |
+/- | 1224/3.4x1010 | |
M. capricolum subsp. capricolum | L15937 | Goat | Contagious agalactia | 1.6+/-0.2x109 | 2.3+/-0.4x108 | (48h) 8.6+/-0.3x106 (96h) 1.1+/-0.6x105 |
+ | 0/8.6x108 | |
Hominis | M. agalactiae | L14628 | Alpine ibex | Pneumonia | 3.9+/-0.2x108 | 3.5+/-0.8x109 | (96h) 5.6+/-0.3x108 | + | UC/5.6x1010 |
5632 | Goat | Contagious agalactia | 1.5+/-0.2x109 | 2.6+/-0.4x108 | (96h) 2.2+/-0.0x108 | + | 624/1.1x1011 | ||
M. fermentans | PG18T | Human | Found associated with various diseases and clinical syndromes | 2.1+/-0.3x108 | 4.4+/-0.6x108 | (96h) 2.0+/-0.0x106 | +++ | 0/2.0x108 | |
M. bovis | L15762 | Cattle | Bovine respiratory complex disease | 9.3+/-1.5x108 | 2.0+/-0.5x109 | (96h) 1.9+/-0.1x106 | + | 1068/1.9x108 |
a Data correspond to mean +/- standard deviation of 3 cell counts, except for M. mycoides subsp. mycoides for which data correspond to mean +/- standard deviation of the three batches i.e. 9 cell counts.
b EV density in electron micrographs was estimated using the following scale: +/-, counts <1 EV/ 5μm2; +, 1–10 EV/ 5μm2; ++, 10–100 EV/ 5μm2; +++ >100 EV/ 5μm2.
c Total number of CFU in EV extract / number of CFU at 48h or 96h (CFU/ml x volume of m-PPLO medium used for EV preparation)
d the non-capsulated variant TR was used in this study (see Material and methods)
UC, uncountable; T, type strain.