Table 1. List and main characteristics of strains used for EV preparation.

Follow-up and quality control of EV production.

Phylogenetic groups	Species/subspecies	Strains	Main hosts	Clinical signs	CFU/ml in m-PPLOa			EV preparation
					0h	24h	48h or 96h	Densityb	Cell contaminationc
Phytoplasma, Acholeplasma	A. laidlawii	PG8T	Vertebrate and invertebrate	none	2.1+/-0.6x109	1.0+/-0.8x109	(96h) 3.5+/-2.4x107	+	56/1.8x1010
Spiroplasma	M. mycoides subsp. mycoides	Afadéd	Cattle	Contagious bovine pleuropneumonia	9.8+/-1.7x108	3.2+/-0.8x109	(96h) 4.9+/-4.3x107	++	0/2.5x1010
	M. mycoides subsp. capri	PG3T	Goat	Contagious agalactia	8.4+/-0.4x108	4.8+/-0.9x109	(48h) 3.4+/-0.0x108 (96h) 9.5+/-2.2x104	+/-	1224/3.4x1010
	M. capricolum subsp. capricolum	L15937	Goat	Contagious agalactia	1.6+/-0.2x109	2.3+/-0.4x108	(48h) 8.6+/-0.3x106 (96h) 1.1+/-0.6x105	+	0/8.6x108
Hominis	M. agalactiae	L14628	Alpine ibex	Pneumonia	3.9+/-0.2x108	3.5+/-0.8x109	(96h) 5.6+/-0.3x108	+	UC/5.6x1010
		5632	Goat	Contagious agalactia	1.5+/-0.2x109	2.6+/-0.4x108	(96h) 2.2+/-0.0x108	+	624/1.1x1011
	M. fermentans	PG18T	Human	Found associated with various diseases and clinical syndromes	2.1+/-0.3x108	4.4+/-0.6x108	(96h) 2.0+/-0.0x106	+++	0/2.0x108
	M. bovis	L15762	Cattle	Bovine respiratory complex disease	9.3+/-1.5x108	2.0+/-0.5x109	(96h) 1.9+/-0.1x106	+	1068/1.9x108

^a Data correspond to mean +/- standard deviation of 3 cell counts, except for M. mycoides subsp. mycoides for which data correspond to mean +/- standard deviation of the three batches i.e. 9 cell counts.

^b EV density in electron micrographs was estimated using the following scale: +/-, counts <1 EV/ 5μm²; +, 1–10 EV/ 5μm²; ++, 10–100 EV/ 5μm²; +++ >100 EV/ 5μm².

^c Total number of CFU in EV extract / number of CFU at 48h or 96h (CFU/ml x volume of m-PPLO medium used for EV preparation)

^d the non-capsulated variant TR was used in this study (see Material and methods)

UC, uncountable; T, type strain.