Fig 3. Box plot of normalized spectral intensities of significantly varying phosphopeptides found in controls (n = 10) vs. babesiosis (n = 10).

Serum amyloid A (SAA) phosphorylated at serine 166 (pS166), kininogen 1 phosphorylated at threonine 326 (pT326), Fibrinogen α phosphorylated at threonine 20 (pT20) and at serine 22 (pS22) are shown for samples from controls (C) and samples from dogs suffering from babesiosis (B). p-value for the comparison C vs. B was calculated using Mann-Whitney test and it is shown for each phosphopeptide. Sequences corresponding to each peptide can be found in Table 1. Normalized phosphopeptide spectral intensity = Spectral intensity of phosphorylation site/Total spectral intensity of the sample.