Fig 5. Intensity ratios pool B/pool H for enriched phosphopeptides compared to pool B/ pool H ratios of total peptide intensities in non-bound and in non-enriched pools.

B/H ratios of phosphopeptide intensity in enriched pools for the corresponding protein is indicated in blue. B/H ratios for intensities of peptides not bound to TiO₂ are shown in red and ratios for peptides observed in pools with no enrichment are shown in green. For some proteins not a single peptide was detected in non-bound or non-enriched (direct) pools. Proteins with higher ratios of enriched phosphopeptides than non-bound and non-enriched peptides are highlighted.