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Split-dCas9 system using dual-AAV vectors. dCas9 is split into two parts, N-terminal lobe with N-split-intein (Cas9N) and C-terminal lobe with C-split-intein (Cas9C). Cas9C is then fused to an effector domain, while Cas9N is linked to the truncated gRNAs for packaging into two separate AAV vectors. Co-delivery and co-expression of these two AAV vectors in vivo will reconstitute the full-length and function of dCas9 fusion protein.
