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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Transgenic Res. 2018 Oct 4;27(6):489–509. doi: 10.1007/s11248-018-0096-8

Figure 3.

Figure 3.

Epigenome editing and transcriptional modulation using transgenic mice or flies. (A) Cre-inducible dCas9-expressing mice. To express the sgRNA and dCas9 fusion protein in vivo, Cre recombinase and sgRNAs are packaged into the adeno-associated virus (AAV) vectors for co-delivery into the Cre-inducible dCas9 fusion protein transgenic mouse. (B) Genetic cross between dCas9- and sgRNA-expressing flies. To express sgRNA and dCas9 fusion protein in vivo, the dCas9 fusion protein and a homozygous sgRNA transgenic flies are genetic crossed.