Table 5.

qPCR analysis of sorted rod photoreceptor and non-rod photoreceptor samples.

Sorted samples		Cycle threshold (Ct) values1
		rho	opn1lw1	opn1lw2	nr2e3	18s
14dpf (larval) xops:eGFP retinas	GFP+samples	19.86	No transcript detected3	No transcript detected	24.39	18.98
	GFP−samples	No transcript detected	22.77	22.18	26.92	18.42
30dpf (juvenile) xops:eGFP retinas	GFP+samples	20.37	No transcript detected	No transcript detected	25.39	18.19
	GFP−samples	No transcript detected	21.66	20.89	28.76	17.88
14dpi (regenerated) xops:eGFP retinas	GFP+samples	18.65	No transcript detected	No transcript detected	25.39	16.95
	GFP−samples	No transcript detected	20.12	19.74	28.52	16.42
30dpi (regenerated) xops:eGFP retinas	GFP+samples	19.30	No transcript detected	No transcript detected	26.41	17.01
	GFP−samples	No transcript detected	21.35	20.53	28.72	16.56

¹Higher Ct values correspond with the presence of less transcript.

²Transcript unreliably detected = transcript quantities approach single copy level (Ct = 34–40).

³No transcript detected = cycle threshold was “UND” (undetermined) by the instrument.