KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Bacterial and Virus Strains | ||
| HIV-1NL4-3 |
Adachi et al., 1986 NIH AIDS Reagent Program |
Cat#114 |
| HIV-2EHO | Rey et al., 1989 | N/A |
| Biological Samples | ||
| One Shot MAX Efficiency DH5α-T1R Competent Cells | ThermoFisher Scientific | Cat#12297016 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| 4ʹ-Ethynyl-2-fluoro-2ʹ-deoxyadenosine (EFdA/MK-8591) | Nakata et al. 2007 | N/A |
| 4ʹ-cyano-2-amino-2ʹ-deoxyadenosine (CAdA) | Kohgo et al., 2004 | N/A |
| 4ʹ-fluoromethyl-2-amino-2ʹ-deoxyadenosine (FMAdA) | Kohgo et al., 2018 | N/A |
| 4ʹ-fluoromethyl-2-fluoro-2ʹ-deoxyadenosine (FMFdA) | This paper | N/A |
| 4ʹ-methyl-2-amino-2ʹ-deoxyadenosine (MAdA) | Kohgo et al., 2018 | N/A |
| 4ʹ-vinyl-2-amino-2ʹ-deoxyadenosine (VAdA) | Kohgo et al., 2018 | N/A |
| 4ʹ-ethyl-2-amino-2ʹ-deoxyadenosine (EtAdA) | Kohgo et al., 2018 | N/A |
| 4ʹ-ethyl-2ʹ-deoxyguanosine (EtdG) | Kohgo et al., 2018 | N/A |
| 4ʹ-methyl-2ʹ-deoxyguanosine (MdG) | Kohgo et al., 2018 | N/A |
| 4ʹ-fluoromethyl-2-amino-N6-methyl-2ʹ-deoxyadenosine (FMAMdA) | This paper | N/A |
| 4ʹ-ethynyl-2ʹ-deoxyguanosine (EdG) |
Ohrui et al. 2000. Kitano et al. 2000. |
N/A |
| 4ʹ-ethynyl-2ʹ-deoxyinosine (EdI) | Ohrui et al. 2000. | N/A |
| 4ʹ-fluoromethyl-2ʹ-deoxycytidine (FMdC) | Kitano et al., 1997 | N/A |
| 4ʹ-ethynyl-2ʹ-deoxycytidine (EdC) |
Kohgo et al., 1999a Kohgo et al., 1999b |
N/A |
| 4ʹ-cyano-2ʹ-deoxythymidine (CdT) | O-Yang et al., 1992 | N/A |
| Critical Commercial Assays | ||
| Cell Counting Kit-8 | Dojindo | Cat#CK04 |
| Lumipulse G1200 | Fujirebio Diagnostics, Inc. | https://www.fujirebio-us.com/Lumipulse/ |
| colorimetric RT assay | Roche Applied Science | Cat#11468120910 |
| Experimental Models: Cell Lines | ||
| MT-4 cells | NIH AIDS Reagent Program | Cat#120 RRID: CVCL_2632 |
| HEK293T cells | ATCC | ATCC CRL-3216 RRID: CVCL_0063 |
| Oligonucleotides | ||
| pNL4-3 | NIH AIDS Reagent Program | Cat#114 |
| Mutagenesis primers | See Table S3 for primer sequence | N/A |
| Recombinant DNA | ||
| HIV-1EFdAR | Maeda et al. 2014 | N/A |
| Software and Algorithms | ||
| Maestro version 10.7.015 | Schrödinger, LLC | https://www.schrodinger.com/maestro/ |