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Agarose gel (1.5%) showing PCR amplified product from CPV-2 vaccine and field strains. Lane M: 100 bp plus DNA ladder (Thermo Scientific, USA), Lane 1–3, 8–10: CPV-2 vaccine strains, Lane 4–6, 11–13: CPV-2 field strains, Lane 7: PCR amplification from healthy unvaccinated dog, Lane 14: Non-template control
