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. 2018 Oct 20;9(5):43–54.

Figure 2.

Figure 2

CENP-E transcript variant one and two are expressed in cancer cell lines. A. Agarose gel showed expression in housekeeping gene RPL4 in 22 cancer cell lines that include: 1 (NIH-OVCAR-3), 2 (SK-OV-3), 3 (TOV-21G), 4 (TOV-112D), 5 (HeLa), 6 (SiHa), 7 (CaLo), 8 (RoVa), 9 (ViBo), 10 (C-33A), 11 (Vipa), 12 (INBL), 13 (Caski), 14 (MS-751), 15 (MDA-MB-231), 16 (MCF-7), 17 (Hep-G2), 18 (HaCat), 19 (Hek-293T), 20 (REH), 21 (Jurkat), 22 (K-562). B. The agarose gel is showing the co-expression of two CENP-E transcripts using consensus primers in 22 cancer cell lines. C. Electropherogram shows the sequence of alternative splice, the top lines indicate the boundaries of partial alternative skipping of exon 38. D. Model of alternative 3’ splice site form exon 38 in CENP-E.