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. 2018 Nov 28;8:255. doi: 10.1038/s41398-018-0300-x

Fig. 4. Glucocorticoid signaling regulates metabolic status of astrocytes.

Fig. 4

a Abundance of indicated transcripts in purified astrocytes obtained from hippocampi collected from CTRL (gray bars) or GRastroKO (blue bars) mice 2 h (left) or 4 h (right) after i.p. administration of DEX (4 mg/kg) (mean ± SEM, *p < 0.05, compared to respective SAL group; n = 3 for 2 h and n = 4 for 4 h timepoint; t test). b Abundance of the Sgk1_001, Sgk1_002 and Sgk1_003 isoforms in homogenates of hippocampi obtained from naïve mice or 2 h after the exposure to quintuple foot shock (5xFS) (mean ± SEM, *p<0.05, **p<0.01, t-test). c Analysis of the effect of SGK1 knockdown on basal and DEX-induced alterations of glucose uptake, lactate release, and glycogen content in primary mouse astrocytes transduced either with LV-shCtrl or LV-shSgk1 and exposed for 24 h to vehicle or DEX (100 nM), normalized to LV-shCtrl Veh (mean ± SEM, *p<0.05, **p<0.01, n = 3 for each group, except in glycogen content (n = 4), two-way ANOVA with Bonferroni post-hoc test)