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. 2018 Nov 28;8:17453. doi: 10.1038/s41598-018-36055-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Titration of the gB-BiTE antibody construct. (a) Flow cytometric analysis of the amounts of BiTE antibody construct bound to T cells, which were previously activated and expanded by anti-CD3/CD28-antibody coated beads. (b) Normalized levels of IFN-γ secreted by activated or resting T cells, respectively, upon co-culture with non-infected or HCMV-infected HFF (AD169, MOI 5, day 4 p.i.) and an increasing amount of the BiTE antibody construct (mean ± standard deviation; n = 3). (c) Normalized levels of TNF secreted by CD3/CD28-activated T cells upon co-culture with non-infected or HCMV-infected HFF (AD169, MOI 5, day 4 p.i.) and an increasing concentration of the BiTE antibody construct (mean ± standard deviation; n = 3). IFN-γ and TNF levels were normalized for each donor to the value obtained with activated T cells co-cultured with HCMV-infected HFF and 1000 ng/ml BiTE antibody construct.