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. 2018 Nov 28;8:17453. doi: 10.1038/s41598-018-36055-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of the efficiency of redirecting T cells to gB by either the BiTE antibody construct or a CAR. Activated T cells were redirected by either the gB-BiTE antibody construct or a gB-CAR and co-cultured with 293T cells, which were electroporated with increasing amounts of chimeric EpCAM-gB mRNA, as indicated. (a) Expression levels of EpCAM-gB in the target cells 293T 18 hours after electroporation with different amounts of EpCAM-gB mRNA. (b) Expression of the gB-CAR in CD3/CD28-activated primary T cells of one representative experiment. (c) Proportion of degranulating T cells as determined by flow cytometric analysis of cell surface expression of CD107a. (d) Normalized levels of secreted IFN-γ (values obtained with gB-BiTE antibody construct at 10 µg EpCAM-gB mRNA were set to 100%). (c,d) display the mean values ± standard deviation of experiments with three donors.