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. 2018 Apr 17;25(12):2181–2194. doi: 10.1038/s41418-018-0109-4

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© ADMC Associazione Differenziamento e Morte Cellulare 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — GRP78 expression in cardiac myocytes. a Immunofluorescence staining showed GRP78 expression in cardiac myocytes in both embryonic and adult (12 week old) hearts. α-Actinin is a marker for cardiac myocyte. Note that GRP78 is also expressed in α-actinin-negative, non-cardiac tissues. Scale bar: 40 μm. b GRP78 expression was decreased in adult compared to embryonic hearts as assessed by qPCR, which was normalized to 18s rRNA. Embryonic indicates 12–15 days post coitum, and adult indicates 3 months old. N = 6–7. c GRP78 protein level was reduced in the adult heart compared to embryonic stage as shown by immunoblotting. N = 6. *p < 0.05