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. 2018 Nov 28;8:17435. doi: 10.1038/s41598-018-35753-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Localization of ribosomes on PABP. (A) A schematic representation of PABP and RRM-deletion mutants. (B) C-terminally His-PA–tagged wild-type PABP (1-2-3-4-C), RRM-deletion mutants and GST proteins (1 μg each, Coomassie brilliant blue stained). (C) and (D) Binding of the 40S ribosomal subunit (C) and the 60S subunit (D) to PABP (1-2-3-4-C) or its deletion mutants immobilized on the TALON resin. Input: one twentieth of the ribosomal sample was analyzed at the same time.