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. 2018 Nov 28;8:17435. doi: 10.1038/s41598-018-35753-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Functional analysis of PABP interaction with the ribosome. (A) Cap-Rluc-A RNA (0.1 μM) was translated in the reconstitution system in the presence of increasing concentrations (0 to 3.84 μM) of PABP (1-2-3-4-C) or a truncated PABP (1-2-C). At indicated times, an aliquot of each sample was removed for the Rluc assay. Each bar represents the mean of two experiments. (B) Cap-Rluc-A RNA (0.1 μM) was translated in the reconstitution system with PABP or PABP (1-2-C) (3.84 μM each) in the presence of increasing concentrations (0 to 7.68 μM) of Paip2 for 6 h. After translation, Rluc activity was measured. Each column and bar represent the mean and standard deviation of three experiments, respectively.