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. 2018 Nov 22;9:1343. doi: 10.3389/fphar.2018.01343

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Copyright © 2018 Ye, Duan, Deng, Wang, Chen, Cui, Sun, Zhang, Du, Gu, Lin and Tang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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EGF promotes migration of BGC-823 by activating Rab35. (A) BGC-823 cells were serum-starved for 12 h and treated with EGF for the indicated concentration or (B) time. The endogenous activated Rab35 was precipitated by GST-RBD35. The expression of Rab35 was analyzed by western blot. (C) BGC-823 cells were transfected with Rab35 siRNAs pool or negative control siRNA (NC). Then, cells were lysed and analyzed for expression of Rab35 and GAPDH. (D) Images were taken at time 0 h and after 18 h or (E) 24 h of wounding. The relative migration rate was calculated by normalizing the values obtained for the NC or GFP group at 18 or 24 h as 100%. All experiments were repeated at least three times (^∗∗P < 0.01; ^∗∗∗P < 0.001).