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. 2018 Nov 22;9:1343. doi: 10.3389/fphar.2018.01343

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Copyright © 2018 Ye, Duan, Deng, Wang, Chen, Cui, Sun, Zhang, Du, Gu, Lin and Tang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The binding of Grb2 and DENND1A affects the activity of Rab35 and the migration of BGC823 cells. (A) BGC-823 cells transfected with or without Flag-Grb2-ΔSH3-N-C were treated with or without EGF. The expression of endogenous activated and total Rab35 was analyzed by western blot. (B) And, the relative migration rate was calculated by normalizing the values obtained for the group of BGC-823 cells treated without Flag-Grb2-ΔSH3-N-C and EGF at 24 h as 100%. (C) Transwell assay was performed on BGC-823 cells transfected with or without Flag-Grb2-ΔSH3-N-C to evaluate the ability of migration and invasion. All experiments were repeated at least three times (^∗∗P < 0.01; ^∗∗∗P < 0.001).