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. 2018 Nov 22;9:685. doi: 10.3389/fendo.2018.00685

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Copyright © 2018 Bardóczi, Wilheim, Skrapits, Hrabovszky, Rácz, Matolcsy, Liposits, Sliwowska, Dobolyi and Kalló.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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GnRH-IR varicosities (green) establish multiple appositions on TH-IR neurons (red) also in the Arc. (a–c) Axo-dendritic and axo-somatic appositions are indicated by arrowheads and arrows; (b,c) are snapshots from the 3D reconstructed and rotated image of the boxed area in (a). The presence of asymmetric synapses (d; arrowheads) were confirmed also by the arcuate appositions. (d)” The GnRH-IR axon terminal contained both dense-core granules (arrows, heavily labeled with SGI-NiDAB) and round-shaped, small, clear vesicles, as shown in the high power image of the boxed area of (d)'. Scale bar in (a) is 100 μm; in (b) and (c) 10 μm; in (d') it is 500 nm.