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Immunohistochemical triple labeling for GnRH (green), TH (blue) and KP (red) in the POA, where the majority of TH neurons are also immunoreactive for KP (a; co-localization in purple). Besides double-labeled neurons (arrowheads), single-labeled for TH (arrows in b) or KP (arrow in c) are also present. The GnRH-IR varicosities form appositions (arrows) on all three phenotypes of neurons (d1–d3). Scale bar in (a) is 50 μm; in (bgb–d3) 10 μm.
