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Immunohistochemical triple labeling for GnRH (green), TH (blue) and KP (red) in the Arc, where TH neurons are not immunoreactive for KP (a); TH- and KP-IR neurons form two separate populations. The TH-IR neurons (arrowheads) receive axo-somatic and axo-dendritic appositions from GnRH-IR fibers (arrows; b,c). rgb; red-green-blue channels. Scale bar in (a) is 50 μm; in (bgb-crgb) 10 μm.
