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Characterization of preoptic and arcuate TH-IR neurons for kisspeptin expression and GnRH-IR afferentation. (a) Percentage of neurons immunoreactive (IR) for tyrosine hydroxylase (TH) and/or kisspeptin (KP) in the preoptic area (POA). (b) Percentage of the GnRH appositions on each of this neuronal phenotype in the POA. (c) The mean number of GnRH appositions on 100 TH-IR neurons in the POA and arcuate nucleus (Arc). Asterisks mark significant changes.
