Fig. 3.

Selective expansion of human myeloid cells and NK cells in humanized mice. a Immune system reconstitution in NRG-HIS mice. Frequency of each cell fraction is shown as a percentage of CD45+ cells, with the exception of CD4+ and CD8+ T cells, which are displayed as a percentage of CD3+ T cells. The frequencies of important myeloid subsets (CD14+ monocytes and CD11c+ dendritic cells) and CD56+ NK cells are highlighted by a red box. Medians are shown for each cell subset frequency as horizontal black line (n = 82). b Schematic representation of the experimental procedure employed to evaluate the ability of NRGF-HIS mice to selectively expand human myeloid cell subsets. 10¹¹ AdV-Fluc or 10¹⁰ AdV-Flt3LG particles were injected into NRG-HIS or NRGF-HIS mice, and immune cell expansion was examined at day 5 and 10 post Adv-injection. c, d Expansion of human immune cell subsets in the spleen (c), bone marrow (c) or blood (d) of NRG-HIS and NRGF-HIS mice. NRG-HIS (blue circle) and NRGF-HIS (red square) mice were injected with AdV-Fluc (closed circle/square in panel c) or AdV-Flt3LG (open circle/square in panel c). e Expansion of murine cDCs and pDCs in the spleen and bone marrow of NRG-HIS and NRGF-HIS mice following injection with AdV-Flt3LG or AdV-Fluc. For panels (c–e), medians are shown as horizontal black lines for each experimental condition (n = 4 per group). *p ≤ 0.05, ns non-significant (Wilcoxon–Mann–Whitney test). cDCs conventional dendritic cells, pDCs plasmacytoid dendritic cells, NK natural killer cells