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. 2018 Nov 22;9:2871. doi: 10.3389/fmicb.2018.02871

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Copyright © 2018 Martínez-Servat, Yero, Huedo, Marquez, Molina, Daura and Gibert.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of hetero-resistant subpopulations of selected S. maltophilia isolates by population analysis profile (PAP). PAP was determined at 24 h by agar dilution of cultures exposed to serial dilutions of colistin. The y axis indicates the number of colonies on MHA plates, and concentrations of colistin are shown on the x axis. Values are means of three or four replicate experiments with SD. The limit of counting was 20 CFU/ml, and it is indicated with a horizontal dashed line. For each strain, MIC value calculated by the BMD method is indicated with a solid vertical blue line and a purple bar indicates the resazurin-stained transition zone.