Skip to main content
. 2018 Nov 28;9:5051. doi: 10.1038/s41467-018-07360-1

Fig. 2.

Fig. 2

Genetic deletion of miR-141 suppresses spontaneous and surgically induced IDD. a Safranin O staining of intervertebral disc from 6-month-old mice (WT, n = 10; miR-141 KO, n = 10), 14-month-old mice (WT, n = 10; miR-141 KO, n = 10), 18-month-old mice (WT, n = 10; miR-141 KO, n = 10), and 22-month-old mice (WT, n = 10; miR-141 KO, n = 10). Scale bar = 50 μm. b The histological grades evaluated at 6, 14, 18, and 22 months in WT and miR-141 KO mice. ***p < 0.001 by unpaired two-sample Student’s t test. c HE and Safranin O staining of disc sections in WT and miR-141 KO mice obtained at 6 and 12 weeks after surgery (WT, n = 10; miR-141 KO, n = 10). HE, scale bar = 50 μm; Safranin O, scale bar = 50 μm. d Histological score of WT and miR-141 KO mice, as measured through Safranin O result. ***p < 0.001 unpaired two-sample Student’s t test. e Immunostaining for collagen II and MMP13 in discs of WT and miR-141 KO mice. Scale bar = 100 μm. Data shown as mean and error bar represents s.e.m.