Table 2.

Standardized relative amount of gga-miR-130b-3p in IMM.

Brain region	IMM, left and right combined		Left IMM		Right IMM
Housekeeper	miR-221-3p	miR-99a-5p	miR-221-3p	miR-99a-5p	miR-221-3p	miR-99a-5p
Untrained chicks
Mean	0.97	1.09	1.01	1.08	0.93	1.11
s.e.m.	0.04	0.05	0.05	0.06	0.04	0.08
df	10	10	10	10	10	10
Trained chicks
Correlation, mi-RNA amount vs preference score	−0.84	−0.84	−0.90	−0.70	−0.69	−0.69
df	10	10	10	10	10	10
P	0.0006*	0.0007*	0.0001*	0.012*	0.013*	0.013*
y-intercept at preference score 100	0.86	1.02	0.82	1.00	0.90	1.03
SE of y-intercept	0.05	0.03	0.04	0.03	0.07	0.04
Comparison, y-intercept at preference score 100 vs mean for untrained
t	−1.66	−1.31	−2.98	−1.17	−0.40	−0.90
df	18.2	16.6	20.0	15.6	16.7	15.5
P	0.11	0.21	0.01*	0.26	0.69	0.38
y-intercept at preference score 50	1.04	1.15	1.05	1.14	1.03	1.16
SE of y-intercept	0.05	0.03	0.04	0.03	0.07	0.04
Comparison, y-intercept at preference score 50 vs mean for untrained chicks
t	1.04	0.97	0.70	1.01	1.18	0.59
df	16.2	19.5	18.3	19.7	15.2	19.7
P	0.31	0.34	0.49	0.33	0.26	0.56
Residual regression variance/variance (untrained)	0.21	0.07	0.15	0.17	0.23	0.08
P	0.020*	0.00030*	0.0056*	0.010*	0.030*	0.00035*

Summary of results of regression of gga-miR-130b-3p in IMM on preference score, both housekeepers. Results are also given for untrained chicks. The bottom two rows test whether residual variance from regression with preference score is significantly different from variance of untrained chicks (probabilities are two-tailed). Statistically significant probabilities are starred. In this and the following tables, and in Supplementary Tables S2–S4, numbers of degrees of freedom have been corrected for differences in sample variances.